Autoria: CECHIN, J.; PIASECKI, C.; BENEMANN, D. P.; KREMER, F. S.; GALLI, V.; MAIA, L. C.; AGOSTINETTO, D.; VARGAS, L.

Resumo: Italian ryegrass (Lolium multiflorum; LOLMU) is one of the most troublesome weeds in temperate regions in the world. This weed species interfere with wheat, corn, rye, and oat, causing significant crop yield losses. This species has evolved glyphosate resistance, making it difcult to control. The mechanisms of glyphosate resistance are still unknown, and an understanding thereof will favor the development of new strategies of management. The present study is the first transcriptome study in LOLMU using glyphosate-resistant and -sensitive biotypes, aiming to identify and to provide a list of the candidate target genes related to glyphosate resistance mechanism. The transcriptome was assembled de novo, producing 87,433 contigs with an N50 of 740 bp and an average length of 575 bp. There were 92 and 54 up- and down-regulated genes, respectively, in the resistant biotype, while a total of 1683 were diferentially expressed in the sensitive biotype in response to glyphosate treatment. We selected 14 highly induced genes and seven with repressed expression in the resistant biotype in response to glyphosate. Of these genes, a significant proportion were related to the plasma membrane, indicating that there is a barrier making it difcult for glyphosate to enter the cell.

Ano de publicação: 2020

Tipo de publicação: Artigo de periódico

Unidade: Embrapa Trigo

Palavras-chave: Diferential gene expression, Italian ryegrass, Next-generation sequencing, RNA-Seq, Resistance mechanism