Autoria: DELLA GIUSTINA, P.; MASTRANGELO, T.; AHMAD, S.; MASCARIN, G. M.; CACERES, C.

Resumo: Abstract: A common strategy used to maintain sterile fly quality without sacrificing sterility is to irradiate the insects under an oxygen-reduced atmosphere. So far, sterilizing doses for the South American fruit fly Anastrepha fraterculus have only been determined under normoxia. Our study reports for the first time the dose-sterility response under hypoxia for two different A. fraterculus strains. The pupae were derived from a bisexual strain (a Brazilian-1 population) and a recently developed genetic sexing strain (GSS-89). Two hours prior to irradiation, pupae were transferred to sealed glass bottles and irradiated when oxygen concentration was below 3%. Four types of crosses with nonirradiated flies of the bisexual strain were set to assess sterility for each radiation dose. For males from both strains, Weibull dose?response curves between radiation doses and the proportion of egg hatch, egg-to-pupa recovery, and recovery of adults were determined. The GSS males revealed high sterility/mortality levels compared to males from the bisexual strain at doses < 40 Gy, but a dose of 74 Gy reduced egg hatch by 99% regardless of the male strain and was considered the sterilizing dose. The fertility of irradiated females was severely affected even at low doses under hypoxia.

Ano de publicação: 2021

Tipo de publicação: Artigo de periódico

Unidade: Embrapa Meio Ambiente

Palavras-chave: Anastrepha Fraterculus, Esterilização, Fruit flies, Irradiation, Lineage, Mosca das Frutas, Oxygen, South american fruit fly, Sterile insect technique