Autoria: NICKEL, O.; FAJARDO, T. V. M.; TARGON, M. L. N. P.; MACHADO, M. A.

Resumo: The coat protein (cp) gene of Apple stem grooving Capillovirus was amplified by RT-PCR, cloned, sequenced and subcloned in the expression plasmid pMal-c2. The ASGV cp gene was expressed in E. coli as a fusion protein (fp) containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP-fp was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fp, gave specific reactions to ASGV from several infected apple cultivars at dilutions of up to 1:2000 in indirect ELISA. The ASGVcp/MBP-fp reacted to antisera raised against it as well as to commercial antisera against ASGV virions in immunoblotting. These results are a fundamental contribution to the development of an efficient ASGVindexing of propagative and mother stock materials in Southern Brazil.

Ano de publicação: 2004

Tipo de publicação: Artigo em anais e proceedings

Unidade: Embrapa Uva e Vinho

Palavras-chave: ASGV, Antibodies, Apples, Elisa, Recombinant antigens, Recombinant viral antigen