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Método de extração de DNA nos estudos de microbiologia ruminal. O objetivo deste documento é apresentar os métodos de extração de DNA ruminal e descrever o protocolo metodológico adotado no projeto RumenGases (01.10.06.001.03.00 SEG, 02.11.05.008.00.00) desenvolvi... ... Repositório: BDPA Ano de publicação: 2018
Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform. Cell-free expression (CFE) systems are one of the main platforms for building synthetic cells. A major drawback is the orthogonality of cell-free systems across species. To generate a CFE system compa... ... Repositório: BDPA Ano de publicação: 2022
Dissecting protein domain variability in the core rna interference machinery of five insect orders. RNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the... ... Repositório: BDPA Ano de publicação: 2021
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. During earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soyb... ... Repositório: BDPA Ano de publicação: 2010

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